Molecular characterization of cotton leaf curl Multan beta-satellite occurance on chili plants in Multan, Pakistan

Molecular characterization of cotton leaf curl Multan beta-satellite occurance on chili plants in Multan, Pakistan

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The prevalence of cotton leaf curl disease (CLCuD) has significantly hampered chili (Capsicum spp.) production, presenting a formidable ProSeries Lighting Parts challenge in Pakistan.During a chili field survey in 2018, distinct symptoms, including stunted growth, yellowing, and severe leaf curling, were observed on several plants.Subsequently, a comprehensive sampling effort was undertaken, collecting a total of 39 symptomatic samples from diverse locations across Multan, Punjab.The DNA extraction from these samples was conducted at the plant virology laboratory at Bahauddin Zakariya University, Multan, marking a crucial step in the investigation of this debilitating disease and its impact on Fishing Gear chili production in the region.

Molecular analysis with PCR using Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33 primers confirmed begomovirus infection in chili plants.Positive amplification demonstrated a 71.79% infection rate, with 579 bp, 1.4 kb, and 481 bp amplicons for Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33, respectively.Sequencing identified cotton leaf curl Multan beta-satellite (MT668934) infecting the chili plant.

Effectively managing these begomoviruses is crucial to curbing their multiplication and protecting vital crops like chili.Addressing the distributions of beta-satellites in agricultural fields, particularly chili, is imperative to prevent further viral spread.